Knockout of pprM Decreases Resistance to Desiccation and Oxidation in Deinococcus radiodurans.

ABSTRACT: Deinococcus radiodurans has attracted a great interest in the past decades due to its extraordinary resistance to ionizing radiation and highly efficient DNA repair system. Recent studies indicated that pprM is a putative pleiotropic gene in D. radiodurans and plays an important role in radioresistance and antioxidation, but its underlying mechanisms are poorly elucidated. In this study, pprM mutation was generated to investigate resistance to desiccation and oxidative stress. The result showed that the survival of pprM mutant under desiccation was markedly retarded compared to the wild strain from day 7-28. Furthermore, knockout of pprM increases the intercellular accumulation of ROS and the sensibility to H₂O₂ stress in the

SUBMITTER: Zeng Y 

PROVIDER: S-EPMC5574771 | biostudies-literature | 2017 Sep

REPOSITORIES: biostudies-literature