Reference-based RADseq resolves robust relationships among closely related species of lichen-forming fungi using metagenomic DNA.

ABSTRACT: Despite increasing availability of phylogenomic datasets, strategies to generate genome-scale data from organisms involved in symbiotic relationships remains challenging. Restriction site-associated DNA sequencing (RADseq) can effectively generated reduced representation genomic loci. However, when using metagenomic DNA from inseparable symbiotic organisms, RADseq loci may belong to any number of the organisms involved in these intimate associations. In this study, we explored the potential for a reference-based RADseq approach to generate data for lichen-forming fungi from metagenomic DNA extracted from intact lichens. We simulated RAD data from draft genomes of closely related lichenized fungi to test if RADseq can reconstruct robust evolutionary relationships. Subsequently, we generated

SUBMITTER: Grewe F 

PROVIDER: S-EPMC5575168 | biostudies-literature | 2017 Aug

REPOSITORIES: biostudies-literature