Relationship and mechanism of Kv2.1 expression to ADH secretion in rats with heart failure.

ABSTRACT:

Objective

To explore the mechanisms of Kv2.1 on the secretion of ADH in rats with heart failure.

Methods

In the animal study, 70 healthy male SD rats were selected. Ligation of coronary heart failure model surgery was performed in 60 rats and sham surgery was performed in the other 10 rats. Q-PCR was used to detect the mRNA expression of Kv2.1 in hypothalamus and heart. The protein expression of Kv2.1 and ADH was detected by western blot. In the cell culture study, hypothalamic neurons were cultured and divided into 7 groups. The mRNA expression of Kv2.1 and ADH was detected by Q-PCR. The protein expression of Kv2.1, CamKII, phosphorylation SynapsinI, dephosphorylation SynapsinI and ADH was detected by western blot.

Results

Compared with the control group of heart fai

SUBMITTER: Li J 

PROVIDER: S-EPMC5575182 | biostudies-literature | 2017

REPOSITORIES: biostudies-literature