Cell-permeable capsids as universal antigen carrier for the induction of an antigen-specific CD8⁺ T-cell response.

ABSTRACT: Vaccine platforms that can be flexibly loaded with antigens can contribute to decrease response time to emerging infections. For many pathogens and chronic infections, induction of a robust cytotoxic T lymphocytes-mediated response is desirable to control infection. Antigen delivery into the cytoplasm of antigen presenting cells favors induction of cytotoxic T cells. By fusion of the cell-permeable translocation motif (TLM)-peptide to the capsid-forming core protein of hepatitis B virus, and by insertion of the strep-tag in the spike tip (a domain that protrudes from the surface of the capsid), cell-permeable carrier capsids were generated that can be flexibly loaded with various antigens. Loading with antigens was demonstrated by electron microscopy, density gradient centrifugation and su

SUBMITTER: Akhras S 

PROVIDER: S-EPMC5575276 | biostudies-literature | 2017 Aug

REPOSITORIES: biostudies-literature