Improved split fluorescent proteins for endogenous protein labeling.

ABSTRACT: Self-complementing split fluorescent proteins (FPs) have been widely used for protein labeling, visualization of subcellular protein localization, and detection of cell-cell contact. To expand this toolset, we have developed a screening strategy for the direct engineering of self-complementing split FPs. Via this strategy, we have generated a yellow-green split-mNeonGreen2_1-10/11 that improves the ratio of complemented signal to the background of FP_1-10-expressing cells compared to the commonly used split GFP_1-10/11; as well as a 10-fold brighter red-colored split-sfCherry2_1-10/11. Based on split sfCherry2, we have engineered a photoactivatable variant that enables single-molecule localization-based super-resolution microscopy. We have demonstrat

SUBMITTER: Feng S 

PROVIDER: S-EPMC5575300 | biostudies-literature | 2017 Aug

REPOSITORIES: biostudies-literature