A reverse micelle strategy for fabricating magnetic lipase-immobilized nanoparticles with robust enzymatic activity.

ABSTRACT: Enzyme-immobilized nanoparticles that are both catalysis effective and recyclable would have wide applications ranging from bioengineering and food industry to environmental fields; however, creating such materials has proven extremely challenging. Herein, we present a scalable methodology to create Candida rugosa lipase-immobilized magnetic nanoparticles (L-MNPs) by the combination of nonionic reverse micelle method and Fe₃O₄ nanoparticles. Our approach causes the naturally abundant and sustainable Candida rugose lipase to ordered-assemble into nanoparticles with high catalytic activity and durability. The resultant L-MNPs exhibit the integrated properties of high porosity, large surface area, fractal dimension, robust enzymatic activity, good durability, and high ma

SUBMITTER: Yi S 

PROVIDER: S-EPMC5575323 | biostudies-literature | 2017 Aug

REPOSITORIES: biostudies-literature