Comprehensive transcriptome analyses reveal tomato plant responses to tobacco rattle virus-based gene silencing vectors.

ABSTRACT: In plants, virus-induced gene silencing (VIGS) is a popular tool for functional genomic studies or rapidly assessing individual gene functions. However, molecular details regarding plant responses to viral vectors remain elusive, which may complicate experimental designs and data interpretation. To this end, we documented whole transcriptome changes of tomato elicited by the application of the most widely used tobacco rattle virus (TRV)-based vectors, using comprehensive genome-wide analyses. Our data illustrated multiple biological processes with functional implications, including (1) the enhanced activity of miR167 in guiding the cleavage of an auxin response factor; (2) reduced accumulation of phased secondary small interfering RNAs from two genomic loci; (3) altered expression of ~500

SUBMITTER: Zheng Y 

PROVIDER: S-EPMC5575331 | biostudies-literature | 2017 Aug

REPOSITORIES: biostudies-literature