Verification of Abbott 25-OH-vitamin D assay on the architect system.

ABSTRACT:

Objectives

Analytical and clinical verification of both old and new generations of the Abbott total 25-hydroxyvitamin D (25OHD) assays, and an examination of reference Intervals.

Methods

Determination of between-run precision, and Deming comparison between patient sample results for 25OHD on the Abbott Architect, DiaSorin Liaison and AB SCIEX API 4000 (LC-MS/MS). Establishment of uncertainty of measurement for 25OHD Architect methods using old and new generations of the reagents, and estimation of reference interval in healthy Irish population.

Results

For between-run precision the manufacturer claims 2.8% coefficients of variation (CVs) of 2.8% and 4.6% for their high and low controls, respectively. Our instrument showed CVs between 4% and 6.2% for all levels of the

SUBMITTER: Hutchinson K 

PROVIDER: S-EPMC5575431 | biostudies-literature | 2017 Apr

REPOSITORIES: biostudies-literature