Comparison between chaotropic and detergent-based sample preparation workflow in tendon for mass spectrometry analysis.

ABSTRACT: Exploring the tendon proteome is a challenging but important task for understanding the mechanisms of physiological/pathological processes during ageing and disease and for the development of new treatments. Several extraction methods have been utilised for tendon mass spectrometry, however different extraction methods have not been simultaneously compared. In the present study we compared protein extraction in tendon with two chaotropic agents, guanidine hydrochloride (GnHCl) and urea, a detergent, RapiGest™, and their combinations for shotgun mass spectrometry. An initial proteomic analysis was performed following urea, GnHCl, and RapiGest™ extraction of equine superficial digital flexor tendon (SDFT) tissue. Subsequently, another proteomic analysis was performed following extraction wit

SUBMITTER: Ashraf Kharaz Y 

PROVIDER: S-EPMC5575552 | biostudies-literature | 2017 Jul

REPOSITORIES: biostudies-literature