Automated and controlled mechanical stimulation and functional imaging in vivo in C. elegans.

ABSTRACT: C. elegans is a useful genetic model system for investigating mechanisms involved in sensory behavior which are potentially relevant to human diseases. While utilities of advanced techniques such as microfluidics have accelerated some areas of C. elegans sensory biology such as chemosensation, studies of mechanosensation conventionally require immobilization by glue and manual delivery of stimuli, leading to low experimental throughput and high variability. Here we present a microfluidic platform that precisely and robustly delivers a wide range of mechanical stimuli and can also be used in conjunction with functional imaging and optical interrogation techniques. The platform is fully automated, thereby greatly enhancing the throughput and robustness of experiments. We show that the behavi

SUBMITTER: Cho Y 

PROVIDER: S-EPMC5575793 | biostudies-literature | 2017 Jul

REPOSITORIES: biostudies-literature