Coral cell separation and isolation by fluorescence-activated cell sorting (FACS).

ABSTRACT:

Background

Generalized methods for understanding the cell biology of non-model species are quite rare, yet very much needed. In order to address this issue, we have modified a technique traditionally used in the biomedical field for ecological and evolutionary research. Fluorescent activated cell sorting (FACS) is often used for sorting and identifying cell populations. In this study, we developed a method to identify and isolate different cell populations in corals and other cnidarians.

Methods

Using fluorescence-activated cell sorting (FACS), coral cell suspension were sorted into different cellular populations using fluorescent cell markers that are non-species specific. Over 30 different cell markers were tested. Additionally, cell suspension from Aiptasia pallida was al

SUBMITTER: Rosental B 

PROVIDER: S-EPMC5575905 | biostudies-literature | 2017 Aug

REPOSITORIES: biostudies-literature