Circular RNA Expression Profiles Alter Significantly in Mouse Brain After Transient Focal Ischemia.

ABSTRACT:

Background and purpose

Circular RNAs (circRNAs) are a novel class of noncoding RNAs formed from many protein-coding genes by backsplicing. Although their physiological functions are not yet completely defined, they are thought to control transcription, translation, and microRNA levels. We investigated whether stroke changes the circRNAs expression profile in the mouse brain.

Methods

Male C57BL/6J mice were subjected to transient middle cerebral artery occlusion, and circRNA expression profile was evaluated in the penumbral cortex at 6, 12, and 24 hours of reperfusion using circRNA microarrays and real-time PCR. Bioinformatics analysis was conducted to identify microRNA binding sites, transcription factor binding, and gene ontology of circRNAs altered after ischemia.

Resul

SUBMITTER: Mehta SL 

PROVIDER: S-EPMC5575968 | biostudies-literature | 2017 Sep

REPOSITORIES: biostudies-literature