Glucocorticoid attenuates acute lung injury through induction of type 2 macrophage.

ABSTRACT:

Background

Acute lung injury (ALI) and acute respiratory distress syndrome (ARDS) are severe inflammatory lung diseases. Methylprednisolone (MP) is a common drug against inflammation in clinic. In this study, we aim to investigate the protective effect of MP on ALI and potential mechanisms.

Methods

Male BABL/c mice were injected through tail vein using lipopolysaccharide (LPS, 5 mg/kg) with or without 5 mg/kg MP. Lung mechanics, tissue injury and inflammation were examined. Macrophage subsets in the lung were identified by flow cytometry. Macrophages were cultured from bone marrow of mice with or without MP. Then, we analyzed and isolated the subsets of macrophages. These isolated macrophages were then co-cultured with CD4⁺ T cells, and the percentage of regulator

SUBMITTER: Tu GW 

PROVIDER: S-EPMC5576304 | biostudies-literature | 2017 Aug

REPOSITORIES: biostudies-literature