Ribosome signatures aid bacterial translation initiation site identification.

ABSTRACT:

Background

While methods for annotation of genes are increasingly reliable, the exact identification of translation initiation sites remains a challenging problem. Since the N-termini of proteins often contain regulatory and targeting information, developing a robust method for start site identification is crucial. Ribosome profiling reads show distinct patterns of read length distributions around translation initiation sites. These patterns are typically lost in standard ribosome profiling analysis pipelines, when reads from footprints are adjusted to determine the specific codon being translated.

Results

Utilising these signatures in combination with nucleotide sequence information, we build a model capable of predicting translation initiation sites and demonstrate its hig

SUBMITTER: Giess A 

PROVIDER: S-EPMC5576327 | biostudies-literature | 2017 Aug

REPOSITORIES: biostudies-literature