Comparing microbiota profiles in induced and spontaneous sputum samples in COPD patients.

ABSTRACT:

Background

Induced and spontaneous sputum are used to evaluate the airways microbiota. Whether the sputum types can be used interchangeably in microbiota research is unknown. Our aim was to compare microbiota in induced and spontaneous sputum from COPD patients sampled during the same consultation.

Methods

COPD patients from Bergen, Norway, were followed between 2006/2010, examined during the stable state and exacerbations. 30 patients delivered 36 sample pairs. DNA was extracted by enzymatic and mechanical lysis methods. The V3-V4 region of the 16S rRNA gene was PCR-amplified and prepared for paired-end sequencing. Illumina Miseq System was used for sequencing, and Quantitative Insights Into Microbial Ecology (QIIME) and Stata were used for bioinformatics and statistical an

SUBMITTER: Tangedal S 

PROVIDER: S-EPMC5576328 | biostudies-literature | 2017 Aug

REPOSITORIES: biostudies-literature