Characterization of adenoviral transduction profile in prostate cancer cells and normal prostate tissue.

ABSTRACT:

Background

Prostate diseases are common in males worldwide with high morbidity. Gene therapy is an attractive therapeutic strategy for prostate diseases, however, it is currently underdeveloped. As well known, adeno virus (Ad) is the most widely used gene therapy vector. The aims of this study are to explore transduction efficiency of Ad in prostate cancer cells and normal prostate tissue, thus further providing guidance for future prostate pathophysiological studies and therapeutic development of prostate diseases.

Methods

We produced Ad expressing enhanced green fluorescence protein (EGFP), and characterized the transduction efficiency of Ad in both human and mouse prostate cancer cell lines in vitro, as well as prostate tumor xenograft, and wild-type mouse prostate tissue

SUBMITTER: Ai J 

PROVIDER: S-EPMC5576497 | biostudies-literature | 2017 Sep

REPOSITORIES: biostudies-literature