Structure of a CLC chloride ion channel by cryo-electron microscopy.

ABSTRACT: CLC proteins transport chloride (Cl^-) ions across cellular membranes to regulate muscle excitability, electrolyte movement across epithelia, and acidification of intracellular organelles. Some CLC proteins are channels that conduct Cl^- ions passively, whereas others are secondary active transporters that exchange two Cl^- ions for one H⁺. The structural basis underlying these distinctive transport mechanisms is puzzling because CLC channels and transporters are expected to share the same architecture on the basis of sequence homology. Here we determined the structure of a bovine CLC channel (CLC-K) using cryo-electron microscopy. A conserved loop in the Cl^- transport pathway shows a structure markedly different from that of CLC transpor

SUBMITTER: Park E 

PROVIDER: S-EPMC5576512 | biostudies-literature | 2017 Jan

REPOSITORIES: biostudies-literature