Helicobacter pylori sabA gene is associated with iron deficiency anemia in childhood and adolescence.

ABSTRACT:

Background

Gastric Helicobacter pylori colonization leads to iron deficiency anemia (IDA), especially in children and adolescents. However the pathogenesis is poorly understood.

Objective

We sought to identify specific H. pylori genes involved in IDA development, by comparing bacterial genome-wide expression profiling in patients affected or not.

Methods

H. pylori were isolated from four children with IDA and four from matched controls without IDA. Based on these isolates, cDNA microarrays under iron-replete or depleted conditions were systematically performed to compare gene expression profiles at the whole genome level. Real-time reverse-transcription (RT-) PCR and protein assays were performed for further assessing the profile differentiation of the identified H. p

SUBMITTER: Kato S 

PROVIDER: S-EPMC5576686 | biostudies-literature | 2017

REPOSITORIES: biostudies-literature