Proteolytic cleavage and PKA phosphorylation of α_1C subunit are not required for adrenergic regulation of Ca_V1.2 in the heart.

ABSTRACT: Calcium influx through the voltage-dependent L-type calcium channel (Ca_V1.2) rapidly increases in the heart during "fight or flight" through activation of the β-adrenergic and protein kinase A (PKA) signaling pathway. The precise molecular mechanisms of β-adrenergic activation of cardiac Ca_V1.2, however, are incompletely known, but are presumed to require phosphorylation of residues in α_1C and C-terminal proteolytic cleavage of the α_1C subunit. We generated transgenic mice expressing an α_1C with alanine substitutions of all conserved serine or threonine, which is predicted to be a potential PKA phosphorylation site by at least one prediction tool, while sparing the residues previously shown to be phosphorylated but shown individually n

SUBMITTER: Katchman A 

PROVIDER: S-EPMC5576811 | biostudies-literature | 2017 Aug

REPOSITORIES: biostudies-literature