Cytotoxic protein from the mushroom Coprinus comatus possesses a unique mode for glycan binding and specificity.

ABSTRACT: Glycans possess significant chemical diversity; glycan binding proteins (GBPs) recognize specific glycans to translate their structures to functions in various physiological and pathological processes. Therefore, the discovery and characterization of novel GBPs and characterization of glycan-GBP interactions are significant to provide potential targets for therapeutic intervention of many diseases. Here, we report the biochemical, functional, and structural characterization of a 130-amino-acid protein, Y3, from the mushroom Coprinus comatus Biochemical studies of recombinant Y3 from a yeast expression system demonstrated the protein is a unique GBP. Additionally, we show that Y3 exhibits selective and potent cytotoxicity toward human T-cell leukemia Jurkat cells compared with a pane

SUBMITTER: Zhang P 

PROVIDER: S-EPMC5576819 | biostudies-literature | 2017 Aug

REPOSITORIES: biostudies-literature