Elucidation of potential sites for antibody engineering by fluctuation editing.

ABSTRACT: Target-specific monoclonal antibodies can be routinely acquired, but the sequences of naturally acquired antibodies are not always affinity-matured and methods that increase antigen affinity are desirable. Most biophysical studies have focused on the complementary determining region (CDR), which directly contacts the antigen; however, it remains difficult to increase the affinity as much as desired. While strategies to alter the CDR to increase antibody affinity are abundant, those that target non-CDR regions are scarce. Here we describe a new method, designated fluctuation editing, which identifies potential mutation sites and engineers a high-affinity antibody based on conformational fluctuations observed by NMR relaxation dispersion. Our data show that relaxation dispersion detects impo

SUBMITTER: Yanaka S 

PROVIDER: S-EPMC5577056 | biostudies-literature | 2017 Aug

REPOSITORIES: biostudies-literature