Detection of frequency-dependent endothelial response to oscillatory shear stress using a microfluidic transcellular monitor.

ABSTRACT: The endothelial microenvironment is critical in maintaining the health and function of the intimal layer in vasculature. In the context of cardiovascular disease (CVD), the vascular endothelium is the layer of initiation for the progression of atherosclerosis. While laminar blood flows are known to maintain endothelial homeostasis, disturbed flow conditions including those the endothelium experiences in the carotid artery are responsible for determining the fate of CVD progression. We present a microfluidic device designed to monitor the endothelium on two fronts: the real-time monitoring of the endothelial permeability using integrated electrodes and the end-point characterization of the endothelium through immunostaining. Our key findings demonstrate endothelial monolayer permeability an

SUBMITTER: Sei YJ 

PROVIDER: S-EPMC5577378 | biostudies-literature | 2017 Aug

REPOSITORIES: biostudies-literature