Diagnostic PET Imaging of Mammary Microcalcifications Using ⁶⁴Cu-DOTA-Alendronate in a Rat Model of Breast Cancer.

ABSTRACT: The development of improved breast cancer screening methods is hindered by a lack of cancer-specific imaging agents and effective small-animal models to test them. The purpose of this study was to evaluate ⁶⁴Cu-DOTA-alendronate as a mammary microcalcification-targeting PET imaging agent, using an ideal rat model. Our long-term goal is to develop ⁶⁴Cu-DOTA-alendronate for the detection and noninvasive differentiation of malignant versus benign breast tumors with PET. Methods: DOTA-alendronate was synthesized, radiolabeled with ⁶⁴Cu, and administered to normal or tumor-bearing aged, female, retired breeder Sprague-Dawley rats for PET imaging. Mammary tissues were subsequently labeled and imaged with light, confocal, and electron microscopy to verify mi

SUBMITTER: Ahrens BJ 

PROVIDER: S-EPMC5577624 | biostudies-literature | 2017 Sep

REPOSITORIES: biostudies-literature