Fluorescence and autoradiographic evaluation of tau PET ligand PBB3 to α-synuclein pathology.

ABSTRACT:

Background

The tau PET ligand 2-((1E,3E)-4-(6-([¹¹ C]methylamino)pyridin-3-yl)buta-1,3-dienyl)benzo[d]thiazol-6-ol ([¹¹ C]PBB3) binds to a wide range of tau pathology; however, binding property of PBB3 to non-tau inclusions remains unknown. To clarify whether [¹¹ C]PBB3 binds to α-synuclein pathology, reactivity of PBB3 was assessed by in vitro fluorescence and autoradiographic labeling of brain sections from α-synucleinopathies patients.

Method

Of 10 pure Lewy body disease and 120 multiple system atrophy (MSA) cases in the Mayo Clinic brain bank, we selected 3 Lewy body disease and 4 MSA cases with a range of α-synuclein severity based on the quantitative analysis of α-synuclein burden. PBB3 fluorescence labeling, double or single immunostai

SUBMITTER: Koga S 

PROVIDER: S-EPMC5577807 | biostudies-literature | 2017 Jun

REPOSITORIES: biostudies-literature