Three-dimensional intracellular structure of a whole rice mesophyll cell observed with FIB-SEM.

ABSTRACT:

Background and aims

Ultrathin sections of rice leaf blades observed two-dimensionally using a transmission electron microscope (TEM) show that the chlorenchyma is composed of lobed mesophyll cells, with intricate cell boundaries, and lined with chloroplasts. The lobed cell shape and chloroplast positioning are believed to enhance the area available for the gas exchange surface for photosynthesis in rice leaves. However, a cell image revealing the three-dimensional (3-D) ultrastructure of rice mesophyll cells has not been visualized. In this study, a whole rice mesophyll cell was observed using a focused ion beam scanning electron microscope (FIB-SEM), which provides many serial sections automatically, rapidly and correctly, thereby enabling 3-D cell structure reconstruction.

Met

SUBMITTER: Oi T 

PROVIDER: S-EPMC5577905 | biostudies-literature | 2017 Jul

REPOSITORIES: biostudies-literature