Extensive alternative splicing transitions during postnatal skeletal muscle development are required for calcium handling functions.

ABSTRACT: Postnatal development of skeletal muscle is a highly dynamic period of tissue remodeling. Here, we used RNA-seq to identify transcriptome changes from late embryonic to adult mouse muscle and demonstrate that alternative splicing developmental transitions impact muscle physiology. The first 2 weeks after birth are particularly dynamic for differential gene expression and alternative splicing transitions, and calcium-handling functions are significantly enriched among genes that undergo alternative splicing. We focused on the postnatal splicing transitions of the three calcineurin A genes, calcium-dependent phosphatases that regulate multiple aspects of muscle biology. Redirected splicing of calcineurin A to the fetal isoforms in adult muscle and in differentiated C2C12 slows the timing of

SUBMITTER: Brinegar AE 

PROVIDER: S-EPMC5577920 | biostudies-literature | 2017 Aug

REPOSITORIES: biostudies-literature