Identification of Reference and Biomarker Proteins in Chlamydomonas reinhardtii Cultured under Different Stress Conditions.

ABSTRACT: Reference proteins and biomarkers are important for the quantitative evaluation of protein abundance. Chlamydomonasreinhardtii was grown under five stress conditions (dark, cold, heat, salt, and glucose supplementation), and the OD₇₅₀ and total protein contents were evaluated on days 0, 1, 2, 4, and 6 of culture. Antibodies for 20 candidate proteins were generated, and the protein expression patterns were examined by western blotting. Reference protein(s) for each treatment were identified by calculating the Pearson's correlation coefficient (PCC) between target protein abundance and total protein content. Histone H3, beta tubulin 1 (TUB-1), ribulose-1,5-bisphosphate carboxylase/oxygenase large subunit (RBCL), and mitochondrial F1F0 ATP synthase subunit 6 (ATPs-6)

SUBMITTER: Shi J 

PROVIDER: S-EPMC5578208 | biostudies-literature | 2017 Aug

REPOSITORIES: biostudies-literature