Polyclonal Crypt Genesis and Development of Familial Small Intestinal Neuroendocrine Tumors.

ABSTRACT:

Background & aims

Small intestinal neuroendocrine tumors (SI-NETs) are serotonin-secreting well-differentiated neuroendocrine tumors believed to originate from enterochromaffin (EC) cells. Intestinal stem cell (ISC) are believed to contribute to the formation of SI-NETs, although little is known about tumor formation or development. We investigated the relationship between EC cells, ISCs, and SI-NETs.

Methods

We analyzed jejuno-ileal tissue specimens from 14 patients with familial SI-NETs enrolled in the Natural History of Familial Carcinoid Tumor study at the National Institutes of Health from January 2009 to December 2014. Frozen and paraffin-embedded tumor tissues of different stages and isolated crypts were analyzed by in situ hybridization and immunohistochemistry. Tumo

SUBMITTER: Sei Y 

PROVIDER: S-EPMC5578471 | biostudies-literature | 2016 Jul

REPOSITORIES: biostudies-literature