Intestinal stem cells contribute to the maturation of the neonatal small intestine and colon independently of digestive activity.

ABSTRACT: The murine intestine, like that of other mammalians, continues to develop after birth until weaning; however, whether this occurs in response to an intrinsic developmental program or food intake remains unclear. Here, we report a novel system for the allotransplantation of small intestine and colon harvested from Lgr5 ^{EGFP-IRES-CreERT2/+}; Rosa26 ^rbw/+ mice immediately after birth into the subrenal capsule of wild-type mice. By histological and immunohistochemical analysis, the developmental process of transplanted small intestine and colon was shown to be comparable with that of the native tissues: mature intestines equipped with all cell types were formed, indicating that these organs do not require food intake for development. The intestinal stem cells in transplant

SUBMITTER: Yanai H 

PROVIDER: S-EPMC5578958 | biostudies-literature | 2017 Aug

REPOSITORIES: biostudies-literature