Prolonged AT₁R activation induces Ca_V1.2 channel internalization in rat cardiomyocytes.

ABSTRACT: The cardiac L-type calcium channel is a multi-subunit complex that requires co-assembling of the pore-forming subunit Ca_V1.2 with auxiliary subunits Ca_Vα₂δ and Ca_Vβ. Its traffic has been shown to be controlled by these subunits and by the activation of various G-protein coupled receptors (GPCR). Here, we explore the consequences of the prolonged activation of angiotensin receptor type 1 (AT₁R) over Ca_V1.2 channel trafficking. Bioluminescence Resonance Energy Transfer (BRET) assay between β-arrestin and L-type channels in angiotensin II-stimulated cells was used to assess the functional consequence of AT₁R activation, while immunofluorescence of adult rat cardiomyocytes revealed the effects of GPCR activation on Ca

SUBMITTER: Hermosilla T 

PROVIDER: S-EPMC5578992 | biostudies-literature | 2017 Aug

REPOSITORIES: biostudies-literature