Proteome-wide acetylation dynamics in human cells.

ABSTRACT: Protein acetylation plays a critical role in biological processes by regulating the functions and properties of proteins. Thus, the study of protein acetylation dynamics is critical for understanding of how this modification influences protein stability, localization, and function. Here we performed a comprehensive characterization of protein acetylation dynamics using mass spectrometry (MS) based proteomics through utilization of ¹³C-glucose or D₃-acetate, which are metabolized into acetyl-coA, labeling acetyl groups through subsequent incorporation into proteins. Samples were collected at eight time points to monitor rates and trends of heavy acetyl incorporation. Through this platform, we characterized around 1,000 sites with significantly increasing acetylation tr

SUBMITTER: Kori Y 

PROVIDER: S-EPMC5579049 | biostudies-literature | 2017 Aug

REPOSITORIES: biostudies-literature