Connecting the dots across time: reconstruction of single-cell signalling trajectories using time-stamped data.

ABSTRACT: Single-cell responses are shaped by the geometry of signalling kinetic trajectories carved in a multidimensional space spanned by signalling protein abundances. It is, however, challenging to assay a large number (more than 3) of signalling species in live-cell imaging, which makes it difficult to probe single-cell signalling kinetic trajectories in large dimensions. Flow and mass cytometry techniques can measure a large number (4 to more than 40) of signalling species but are unable to track single cells. Thus, cytometry experiments provide detailed time-stamped snapshots of single-cell signalling kinetics. Is it possible to use the time-stamped cytometry data to reconstruct single-cell signalling trajectories? Borrowing concepts of conserved and slow variables from non-equilibrium statis

SUBMITTER: Mukherjee S 

PROVIDER: S-EPMC5579131 | biostudies-literature | 2017 Aug

REPOSITORIES: biostudies-literature