Zoledronic acid renders human M1 and M2 macrophages susceptible to Vδ2⁺ γδ T cell cytotoxicity in a perforin-dependent manner.

ABSTRACT: Vδ2⁺ T cells are a subpopulation of γδ T cells in humans that are cytotoxic towards cells which accumulate isopentenyl pyrophosphate. The nitrogen-containing bisphosphonate, zoledronic acid (ZA), can induce tumour cell lines to accumulate isopentenyl pyrophosphate, thus rendering them more susceptible to Vδ2⁺ T cell cytotoxicity. However, little is known about whether ZA renders other, non-malignant cell types susceptible. In this study we focussed on macrophages (Mϕs), as these cells have been shown to take up ZA. We differentiated peripheral blood monocytes from healthy donors into Mϕs and then treated them with IFN-γ or IL-4 to generate M1 and M2 Mϕs, respectively. We characterised these Mϕs based on their phenotype and cytokine production and then tested whether Z

SUBMITTER: Fowler DW 

PROVIDER: S-EPMC5579165 | biostudies-literature | 2017 Sep

REPOSITORIES: biostudies-literature