Minisequencing on oligonucleotide microarrays: comparison of immobilisation chemistries.

ABSTRACT: In the microarray format of the minisequencing method multiple oligonucleotide primers immobilised on a glass surface are extended with fluorescent ddNTPs using a DNA polymerase. The method is a promising tool for large-scale single nucleotide polymorphism (SNP) detection. We have compared eight chemical methods for covalent immobilisation of the oligonucleotide primers on glass surfaces. We included both commercially available, activated slides and slides that were modified by ourselves. In the comparison the differently derivatised glass slides were evaluated with respect to background fluorescence, efficiency of attaching oligonucleotides and performance of the primer arrays in minisequencing reactions. We found that there are significant differences in background fluorescence levels am

SUBMITTER: Lindroos K 

PROVIDER: S-EPMC55793 | biostudies-literature | 2001 Jul

REPOSITORIES: biostudies-literature