GT-rich promoters can drive RNA pol II transcription and deposition of H2A.Z in African trypanosomes.

ABSTRACT: Genome-wide transcription studies are revealing an increasing number of "dispersed promoters" that, unlike "focused promoters", lack well-conserved sequence motifs and tight regulation. Dispersed promoters are nevertheless marked by well-defined chromatin structures, suggesting that specific sequence elements must exist in these unregulated promoters. Here, we have analyzed regions of transcription initiation in the eukaryotic parasite Trypanosoma brucei, in which RNA polymerase II transcription initiation occurs over broad regions without distinct promoter motifs and lacks regulation. Using a combination of site-specific and genome-wide assays, we identified GT-rich promoters that can drive transcription and promote the targeted deposition of the histone variant H2A.Z in a genomic

SUBMITTER: Wedel C 

PROVIDER: S-EPMC5579346 | biostudies-literature | 2017 Sep

REPOSITORIES: biostudies-literature