Loss of the canonical spindle orientation function in the Pins/LGN homolog AGS3.

ABSTRACT: In many cell types, mitotic spindle orientation relies on the canonical "LGN complex" composed of Pins/LGN, Mud/NuMA, and Gα_i subunits. Membrane localization of this complex recruits motor force generators that pull on astral microtubules to orient the spindle. Drosophila Pins shares highly conserved functional domains with its two vertebrate homologs LGN and AGS3. Whereas the role of Pins and LGN in oriented divisions is extensively documented, involvement of AGS3 remains controversial. Here, we show that AGS3 is not required for planar divisions of neural progenitors in the mouse neocortex. AGS3 is not recruited to the cell cortex and does not rescue LGN loss of function. Despite conserved interactions with NuMA and Gα_iin vitro, comparison of LGN and A

SUBMITTER: Saadaoui M 

PROVIDER: S-EPMC5579374 | biostudies-literature | 2017 Sep

REPOSITORIES: biostudies-literature