Chromatin stiffening underlies enhanced locus mobility after DNA damage in budding yeast.

ABSTRACT: DNA double-strand breaks (DSBs) induce a cellular response that involves histone modifications and chromatin remodeling at the damaged site and increases chromosome dynamics both locally at the damaged site and globally in the nucleus. In parallel, it has become clear that the spatial organization and dynamics of chromosomes can be largely explained by the statistical properties of tethered, but randomly moving, polymer chains, characterized mainly by their rigidity and compaction. How these properties of chromatin are affected during DNA damage remains, however, unclear. Here, we use live cell microscopy to track chromatin loci and measure distances between loci on yeast chromosome IV in thousands of cells, in the presence or absence of genotoxic stress. We confirm that DSBs result in enh

SUBMITTER: Herbert S 

PROVIDER: S-EPMC5579376 | biostudies-literature | 2017 Sep

REPOSITORIES: biostudies-literature