Development of duplex PCR for differential detection of goatpox and sheeppox viruses.

ABSTRACT:

Background

Clinically, sheeppox and goatpox have the same symptoms and cannot be distinguished serologically. A cheaper and easy method for differential diagnosis is important in control of this disease in endemic region.

Methods

A duplex PCR assay was developed for the specific differential detection of Goatpox virus (GTPV) and Sheeppox virus (SPPV), using two sets of primers based on viral E10R gene and RPO132 gene.

Results

Nucleic acid electrophoresis results showed that SPPV-positive samples appear two bands, and GTPV-positive samples only one stripe. There were no cross-reactions with nucleic acids extracted from other pathogens including foot-and-mouth disease virus, Orf virus. The duplex PCR assay developed can specially detect SPPV or GTPV present in samples (

SUBMITTER: Zhao Z 

PROVIDER: S-EPMC5579950 | biostudies-literature | 2017 Aug

REPOSITORIES: biostudies-literature