Dual peptide-mediated targeted delivery of bioactive siRNAs to oral cancer cells in vivo.

ABSTRACT:

Objectives

Despite significant advances in cancer treatment, the prognosis for oral cancer remains poor in comparison to other cancer types, including breast, skin, and prostate. As a result, more effective therapeutic modalities are needed for the treatment of oral cancer. Consequently, in the present study, we examined the feasibility of using a dual peptide carrier approach, combining an epidermal growth factor receptor (EGFR)-targeting peptide with an endosome-disruptive peptide, to mediate targeted delivery of small interfering RNAs (siRNAs) into EGFR-overexpressing oral cancer cells and induce silencing of the targeted oncogene, cancerous inhibitor of protein phosphatase 2A (CIP2A).

Materials and methods

Fluorescence microscopy, real-time PCR, Western blot analysis, an

SUBMITTER: Alexander-Bryant AA 

PROVIDER: S-EPMC5580030 | biostudies-literature | 2017 Sep

REPOSITORIES: biostudies-literature