Sparse conserved under-methylated CpGs are associated with high-order chromatin structure.

ABSTRACT:

Background

Whole-genome bisulfite sequencing (WGBS) is the gold standard for studying landscape DNA methylation. Current computational methods for WGBS are mainly designed for gene regulatory regions with multiple under-methylated CpGs (UMCs), such as promoters and enhancers.

Results

To reliably predict the functional importance of single isolated UMCs across the genome, which is usually not achievable using traditional methods, we develop a multi-sample-based method. We identified 9421 sparse conserved under-methylated CpGs (scUMCs) from 31 high-quality methylomes, which are enriched in distal interacting anchor regions co-occupied by multiple chromatin-loop factors and are flanked by highly methylated CpGs. Moreover, cell lineage-specific scUMCs are associated with essenti

SUBMITTER: Lin X 

PROVIDER: S-EPMC5580327 | biostudies-literature | 2017 Aug

REPOSITORIES: biostudies-literature