Ratiometric quantitation of redox status with a molecular Fe₂ magnetic resonance probe.

ABSTRACT: We demonstrate the ability of a molecular Fe₂ complex to enable magnetic resonance (MR)-based ratiometric quantitation of redox status, namely through redox-dependent paramagnetic chemical exchange saturation transfer (PARACEST). Metalation of a tetra(carboxamide) ligand with Fe^II and/or Fe^III in the presence of etidronate ion affords analogous FeII2, Fe^IIFe^III, and FeIII2 complexes. Both FeII2 and Fe^IIFe^III complexes give highly-shifted, sharp, and non-overlapping NMR spectra, with multiple resonances for each complex corresponding to exchangeable carboxamide protons. These protons can be selectively irradiated to give CEST peaks at 74 and 83 ppm vs. H₂O for the Fe^IIFe^III c

SUBMITTER: Du K 

PROVIDER: S-EPMC5580376 | biostudies-literature | 2017 Jun

REPOSITORIES: biostudies-literature