Optimization of a metatranscriptomic approach to study the lignocellulolytic potential of the higher termite gut microbiome.

ABSTRACT:

Background

Thanks to specific adaptations developed over millions of years, the efficiency of lignin, cellulose and hemicellulose decomposition of higher termite symbiotic system exceeds that of many other lignocellulose utilizing environments. Especially, the examination of its symbiotic microbes should reveal interesting carbohydrate-active enzymes, which are of primary interest for the industry. Previous metatranscriptomic reports (high-throughput mRNA sequencing) highlight the high representation and overexpression of cellulose and hemicelluloses degrading genes in the termite hindgut digestomes, indicating the potential of this technology in search for new enzymes. Nevertheless, several factors associated with the material sampling and library preparation steps make the metatr

SUBMITTER: Marynowska M 

PROVIDER: S-EPMC5580439 | biostudies-literature | 2017 Sep

REPOSITORIES: biostudies-literature