Soluble epoxide hydrolase activity and pharmacologic inhibition in horses with chronic severe laminitis.

ABSTRACT:

Background

The roles of soluble epoxide hydrolase and lipid mediators in inflammatory and neuropathic pain could be relevant in laminitis pain management.

Objectives

To determine soluble epoxide hydrolase (sEH) activity in the digital laminae, sEH inhibitor potency in vitro, and efficacy of a sEH inhibitor as an adjunct analgesic therapy in chronic laminitic horses.

Study design

In vitro experiments and clinical case series.

Methods

sEH activity was measured in digital laminae from euthanised healthy and laminitic horses (n = 5-6/group). Potency of 7 synthetic sEH inhibitors was determined in vitro using equine liver cytosol. One of them (t-TUCB; 0.1 mg/kg bwt i.v. every 24 h) was selected based on potency and stability, and used as adjunct therapy in 10 horses

SUBMITTER: Guedes A 

PROVIDER: S-EPMC5580818 | biostudies-literature | 2017 May

REPOSITORIES: biostudies-literature