Analysis of Human Endogenous Retrovirus Expression in Multiple Sclerosis Plaques.

ABSTRACT:

Background

It has been suggested that Human endogenous retroviruses (HERVs) are associated with multiple sclerosis (MS) pathogenesis. The objective of this study was to broadly evaluate the expression of HERV core (GAG) and envelope (ENV) genes in diseased brain white matter samples from MS patients compared to normal controls.

Methods

Twenty-eight HERV GAG and 88 ENV gene sequences were retrieved, classified by phylogeny, and grouped into clades. Consensus qPCR primers were designed for each clade, and quantitative PCR was performed on 33 MS and 9 normal control frozen brain samples. MS samples included chronic progressive (n=5), primary progressive (n=4), secondary progressive (n=14), relapsing remitting (n=3) and unclassified confirmed MS cases (n=7). The levels of GAG an

SUBMITTER: Bhetariya PJ 

PROVIDER: S-EPMC5580941 | biostudies-literature | 2017 Aug

REPOSITORIES: biostudies-literature