Opposing effects of in vitro differentiated macrophages sub-type on epithelial wound healing.

ABSTRACT: Inappropriate repair responses to pulmonary epithelial injury have been linked to perturbation of epithelial barrier function and airway remodelling in a number of respiratory diseases, including chronic obstructive pulmonary disease and idiopathic pulmonary fibrosis. We developed an in vitro mechanical scratch injury model in air-liquid interface differentiated primary human small airway epithelial cells that recapitulates many of the characteristics observed during epithelial wound injury in both human tissue and small animal models. Wound closure was initially associated with de-differentiation of the differentiated apical cells and rapid migration into the wound site, followed by proliferation of apical cells behind the wound edge, together with increases in FAK expression, fibronectin

SUBMITTER: Gindele JA 

PROVIDER: S-EPMC5581193 | biostudies-literature | 2017

REPOSITORIES: biostudies-literature