Molecular analysis of HER2 signaling in human breast cancer by functional protein pathway activation mapping.

ABSTRACT:

Purpose

Targeting of the HER2 protein in human breast cancer represents a major advance in oncology but relies on measurements of total HER2 protein and not HER2 signaling network activation. We used reverse-phase protein microarrays (RPMA) to measure total and phosphorylated HER2 in the context of HER family signaling to understand correlations between phosphorylated and total levels of HER2 and downstream signaling activity.

Experimental design

Three independent study sets, comprising a total of 415 individual patient samples from flash-frozen core biopsy samples and formalin-fixed and paraffin-embedded (FFPE) surgical and core samples, were analyzed via RPMA. The phosphorylation and total levels of the HER receptor family proteins and downstream signaling molecules were m

SUBMITTER: Wulfkuhle JD 

PROVIDER: S-EPMC5581198 | biostudies-literature | 2012 Dec

REPOSITORIES: biostudies-literature