Endothelin-1 Stimulates Vasoconstriction Through Rab11A Serine 177 Phosphorylation.

ABSTRACT:

Rationale

Large-conductance calcium-activated potassium channels (BK) are composed of pore-forming BKα and auxiliary β1 subunits in arterial smooth muscle cells (myocytes). Vasoconstrictors, including endothelin-1 (ET-1), inhibit myocyte BK channels, leading to contraction, but mechanisms involved are unclear. Recent evidence indicates that BKα is primarily plasma membrane localized, whereas the cellular location of β1 can be rapidly altered by Rab11A-positive recycling endosomes. Whether vasoconstrictors regulate the multisubunit composition of surface BK channels to stimulate contraction is unclear.

Objective

Test the hypothesis that ET-1 inhibits BK channels by altering BKα and β1 surface trafficking in myocytes, identify mechanisms involved, and determine functional sign

SUBMITTER: Zhai X 

PROVIDER: S-EPMC5581242 | biostudies-literature | 2017 Sep

REPOSITORIES: biostudies-literature