Using an endoscopic distal cap to collect pancreatic fluid from the ampulla (with video).

ABSTRACT:

Background and aims

Duodenal collections of pancreatic fluid can be used as a source of mutations and other markers of pancreatic ductal neoplasia, but admixing pancreatic juice with duodenal contents lowers the concentrations of mutations. Collecting pancreatic fluid directly from the ampulla could yield a purer sample of pancreatic fluid.

Methods

We used an endoscopic distal cap attachment to "cap" the ampulla and collect secretin-stimulated pancreatic fluid samples for 5 minutes from 81 patients undergoing pancreatic evaluation as part of the Cancer of the Pancreas Screening studies. We compared mutation concentrations (K-ras and GNAS) measured by droplet-digital PCR (ddPCR) in "cap-collected juice" samples to those found in juice samples obtained from 77 patients collect

SUBMITTER: Suenaga M 

PROVIDER: S-EPMC5581309 | biostudies-literature | 2017 Dec

REPOSITORIES: biostudies-literature