Angiotensin II subtype 1a receptor signaling in resident hepatic macrophages induces liver metastasis formation.

ABSTRACT: Liver metastases from colorectal cancer (CRC) are a clinically significant problem. The renin-angiotensin system is involved in tumor growth and metastases. This study was designed to evaluate the role of angiotensin II subtype receptor 1a (AT1a) in the formation of liver metastasis in CRC. A model of liver metastasis was developed by intrasplenic injection of mouse colon cancer (CMT-93) into AT1a knockout mice (AT1aKO) and wild-type (C57BL/6) mice (WT). Compared with WT mice, the liver weight and liver metastatic rate were significantly lower in AT1aKO. The mRNA levels of CD31, transforming growth factor- β1 (TGF-β1), and F4/80 were suppressed in AT1aKO compared with WT. Double immunofluorescence analysis showed that the number of accumulated F4/80⁺ cells expressing TGF-β1 in m

SUBMITTER: Shimizu Y 

PROVIDER: S-EPMC5581524 | biostudies-literature | 2017 Sep

REPOSITORIES: biostudies-literature