Intestinal Epithelial Sirtuin 1 Regulates Intestinal Inflammation During Aging in Mice by Altering the Intestinal Microbiota.

ABSTRACT:

Background & aims

Intestinal epithelial homeostasis is maintained by complex interactions among epithelial cells, commensal gut microorganisms, and immune cells. Disruption of this homeostasis is associated with disorders such as inflammatory bowel disease (IBD), but the mechanisms of this process are not clear. We investigated how Sirtuin 1 (SIRT1), a conserved mammalian NAD⁺-dependent protein deacetylase, senses environmental stress to alter intestinal integrity.

Methods

We performed studies of mice with disruption of Sirt1 specifically in the intestinal epithelium (SIRT1 iKO, villin-Cre+, Sirt1^flox/flox mice) and control mice (villin-Cre-, Sirt1^flox/flox) on a C57BL/6 background. Acute colitis was induced in some mice by addition of 2.5% d

SUBMITTER: Wellman AS 

PROVIDER: S-EPMC5581719 | biostudies-literature | 2017 Sep

REPOSITORIES: biostudies-literature